Gel Filtration with Buffers Containing Dextran.
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چکیده
منابع مشابه
Purification of an acid alpha-glucosidase by dextran-gel filtration.
1. The behaviour of rat liver alpha-glucosidases on dextran gel (Sephadex G-100) columns was studied. A ;retardation' of an acid alpha-glucosidase activity was observed. This activity was identified as lysosome alpha-(1-->4)-glucosidase. A single gel-filtration step resulted in a 700-fold purification of the enzyme. The same technique was also used to purify the acid alpha-glucosidase of human ...
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Amer. J. Med. 27,963 (1959). [7] GORDON, R.: Exsudative enteropathy. Lancet 191)9/1, 325. [8] SCHWARTZ, M., and B. TROMSEN: Idiopathic or hypcrcatabolic hypoproteinaemia. Brit. med. J. 191)7 I, 14. [9] BARANDUN, S., R. AEBERsoLD u. R. BIANCRI: "Proteindiarrhoe". Schweiz. med. Wschr. 90, 1458 (1960). [10] HANIOKI,Z., J.HAWIGER, P.HIRSZEL, and Z. TWARDOWSKI: On some aspects of the antibody defici...
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The role of sialic acid in the gel-filtration behaviour of sialoglycoproteins was investigated by using the separated isoenzymes of purified human liver alpha-L-fucosidase and several other well-known sialic acid-containing glycoproteins (fetuin, alpha1-acid glycoprotein, thyroglobulin and bovine submaxillary mucin). For each glycoprotein studied, gel filtration of its desialylated derivative g...
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Gel-filtration chromatography is a versatile method that permits the effective separation of biological molecules in high yield. This article describes the basis of the method, the selection of suitable operating conditions, and contrasts typical matrix types. Applications of the technique are described, with references to the scientific literature.
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Allen, R. J. L. (1940). Biochem. J. 34, 858. Bartlett, P. D., Grimmett, P., Beers, L. & Shelata, S. (1956). J. biol. Chem. 218, 419. Burton, K. (1956). Biochem. J. 62, 315. Caiger, P., Morton, R. K., Jarrett, I. G. & Filsell, 0. H. (1962). Biochem. J. 85, 351. Filsell, 0. H., Jarrett, I. G., Atkinson, M. R., Caiger, P. & Morton, R. K. (1963). Biochem. J. 89, 92. Fishkin, A. F. & Lata, G. F. (19...
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ژورنال
عنوان ژورنال: Acta Chemica Scandinavica
سال: 1965
ISSN: 0904-213X
DOI: 10.3891/acta.chem.scand.19-1791